Sharpening the Microtome Knife

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The sharpening of microtome knives.

The regular production of a satisfactory edge on a microtome knife for the cutting of sections as thin as 2 u, which are free from scores and other blemishes, is a serious technical problem in histological laboratories. Eyen when a good edge is produced on a knife, its life is generally limited because contact with any hard material in the specimen may damage the delicate edge, causing it to sc...

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A microtome knife guard.

The base sledge microtome presents a hazard to the operator due to the length of blade that is exposed. Manipulation of the carriage requires hand movements close to the knife edge and serious lacerations may result. A simple, effective guard has been devised, suitable for knives which have screw holes for handles at both ends. It consists of two perspex end-pieces (Fig. I), attached to the kni...

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Faster Sharpening of Microtome Knives by the Use of Alumina-Dreft Suspension.

Science, a weekly journal, Is published each Friday by the American Association for the Advancement of Science at The Business Press, Incorporated, N. Queen St. and McGovern Ave., Lancaster, Pa. Founded in 1880, It has been since 1900 the official publication of the AAAS. Editorial and Advertising Offices, 1515 Massachusetts Avenue, N.W., Washington 5, D. C. Telephone, EXecutive 0 or 661. Cable...

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Some Effects of the Microtome Knife and Electron Beam on Methacrylate-embedded Thin Sections

A technique for the examination of specimens at low electron beam intensity has been presented. Sections micrographed with this technique showed numerous knife scratches and frequently contained bands running parallel to the knife edge. Banding with an average spacing of 0.2 micro appeared to result from periodic distortion produced by impact of the knife. At the beam intensities customarily em...

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Rotary microtome

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ژورنال

عنوان ژورنال: Nature

سال: 1954

ISSN: 0028-0836,1476-4687

DOI: 10.1038/173958a0